@article {1722, title = {Behavioral, morphological and histopathological effects of sublethal doses of quercetin on the species Polycelis felina (Dalyell)}, journal = {Folia Biol (Krakow)}, volume = {61}, year = {2013}, month = {2013}, pages = {101-6}, abstract = {

The effect of quercetin was studied on the multi-eyed flatworm Polycelis felina (Daly.) in laboratory conditions. This is a very suitable test-organism available year-round in nature, easily maintained in the laboratory. The results showed that depending on the dose and recovery period quercetin caused locomotory (behavioral) changes in treated animals including aggregation in groups, resting and unsynchronized movements and twisting of particular body parts. Morphological changes in the form of depigmentation of certain body parts were the result of damage or deterioration ofreticular and parenchymal cells. Numerous basophilic bodies representing parts of damaged and decomposed cells were determined in the gastroderm between the third and seventh day after treatment. All histological preparations revealed significant deterioration of cellular material, and therefore a damaging effect of quercetin on the multi-eyed flatworm Polycelis felina (Daly.). We found an increased number of neoblasts and reticular cells on the third day after treatment, so we concluded that quercetin in the applied doses had a stimulating effect on cell division of neoblasts and reticular cells of treated flatworms.

}, keywords = {Animals, Behavior, Animal, Motor Activity, Platyhelminths, Quercetin}, issn = {0015-5497}, author = {Rajevi{\'c}, Nives and Matkovi{\'c}, Helena and Gregorovi{\'c}, Gordana and Kovacevi{\'c}, Goran and Knezovi{\'c}, Lejla and Kalafati{\'c}, Mirjana} } @article {1719, title = {Resistance of two planarian species to UV-irradiation}, journal = {Folia Biol (Krakow)}, volume = {54}, year = {2006}, month = {2006}, pages = {103-8}, abstract = {

The aim of this work was to determine the effects of 20, 25 and 30 minute UV-irradiation periods lambda = 253.5 nm to two planarian species Dugesia tigrina (Gir.) and Polycelis felina (Daly.). In vivo, UV light effects have been reported to affect intracellular receptors and disrupt simple behaviour. The effects of UV-rays on mortality and behavior as well as morphological, cytological and histological changes in the two planarian species were assessed, and the course and the dynamics of regenerative processes were compared between them. Experimental populations of Dugesia tigrina and Polycelis felina species were maintained in laboratory conditions at room temperature. Mortality, behavioral and morphological changes were monitored daily by means of a light stereomicroscope. For cytological and histopathological analysis, planarians were fixed in Bouine fixative on the first, second, third, fifth and seventh day after exposure to UV-irradiation, respectively. They were embedded in paraffin, cut on a microtome, stained with toluidin blue and embedded in Canada-balsam. UV-rays caused mortality, behavioral, morphological, cytological and histological changes in each planarian species. In regeneration of damaged body parts reticular cells and neoblasts played the main role. Neoblasts as totipotent cells extremely increased in number in the area of damaged tissue, immediately after UV-exposure. Dugesia tigrina was more sensitive to UV-rays than Polycelis felina due to possession of less pigmented cells. The course of regeneration in both species was similar. Most individuals of both species regenerated in 5 to 12 days after UV-irradiation.

}, keywords = {Animals, planarians, Ultraviolet Rays}, issn = {0015-5497}, author = {Kalafati{\'c}, Mirjana and Kovacevi{\'c}, Goran and Franjevi{\'c}, Damjan} } @article {1716, title = {Karyological analysis of two allopatric populations of planarian Polycelis felina (Daly.) in Croatia}, journal = {Folia Biol (Krakow)}, volume = {52}, year = {2004}, month = {2004}, pages = {131-3}, abstract = {

The results of preliminary karyological investigations of two geographically distant and morphologically slightly different Polycelis felina (Daly.) populations from central Croatia are reported. The results have shown that individuals of both populations are diploids, with the same chromosome number in their neoblasts (2n=18). Their karyotypes were composed of nine chromosome pairs, three of them are metacentric and the other six are submetacentric. Statistical evaluation of data indicated that both populations of Polycelis felina (Daly.), despite minor differences observed between them, belong to the same karyological biotype.

}, keywords = {Animals, Chromosomes, Croatia, Geography, Karyotyping, planarians, Species Specificity}, issn = {0015-5497}, author = {Kalafati{\'c}, Mirjana and Kopjar, Nevenka and Zrna, Gordana and Zupan, Irina and Kovacevi{\'c}, Goran and Franjevi{\'c}, Damjan} }