@article {1708, title = {Anatomical deviation of male organs of land planarians from Rio de Janeiro, Brazil, with description of two new species of Cratera (Platyhelminthes, Tricladida).}, journal = {Zootaxa}, volume = {3931}, year = {2015}, month = {2015 Mar 11}, pages = {27-40}, abstract = {

Two new land planarian species, collected in the State of Rio de Janeiro, Brazil, are described. Their external aspect is similar to that of Imbira marcusi Carbayo et al., 2013 and Pseudogeoplana theresopolitana (Schirch, 1929), respectively. The analysis of the internal organs, however, revealed they belong to the genus Cratera. The male copulatory organs of one species is very different from any other geoplaninid, for the penis papilla holds a large, distal cavity receiving the ejaculatory duct and, furthermore, the papilla projects vertically downwards from the roof of the male atrium. Thus we consider it as a new species, Cratera cuarassu sp. nov. The second species differs from its congeners in that the dorsal insertion of the penis papilla is anterior to the ventral one, and in that the female atrium is narrowed in the anterior portion. The species was found in the type locality of Pseudogeoplana theresopolitana (Schirch, 1929) and compares well with it in the external features. However, since its internal organs are unknown and the type material of the species is seemingly lost, we describe it as Cratera anamariae Carbayo, sp. nov.

}, keywords = {Animals, Brazil, Genitalia, Male, Male, planarians}, issn = {1175-5334}, doi = {10.11646/zootaxa.3931.1.2}, author = {Carbayo, Fernando and Almeida, Ana Laura} } @article {ZSC:ZSC12175, title = {Non-destructive imaging to describe a new species of Obama land planarian (Platyhelminthes, Tricladida)}, journal = {Zoologica Scripta}, volume = {45}, number = {5}, year = {2016}, pages = {566{\textendash}578}, abstract = {

The land planarians in the genus Obama include the largest species of the Neotropical Geoplaninae. Morphological discrimination of Obama species can be difficult, as many species are morphologically similar and some present asymmetric \– difficult to interpret \– copulatory organs. New techniques are thus welcomed to provide faster species description and identification. Here, we study several specimens of Obama by means of mainly 2D and 3D \μCT-based images obtained through X-ray microcomputed tomography (\μCT) of a paratype, and, complementarily, histological sections of the holotype and a second paratype, which were digitized into virtual slides. Comparison of these images with traditional histological sections and descriptions of the known species of the genus allows us to conclude that our specimens constitute a new species, which we describe here. We further evaluate the phylogenetic placement of the new species using gene fragments from the mitochondrial cytochrome c oxidase subunit I and the nuclear elongation factor-1\α. Raw and derivative \μCT data and virtual histological sections were deposited in an open repository (GigaDB) and are freely available. This work leads us to conclude that \μCT constitutes a relatively fast, inexpensive non-destructive method that produces results comparable to those of traditional histology, and is thus amenable for describing flatworm species.

}, issn = {1463-6409}, doi = {10.1111/zsc.12175}, url = {http://dx.doi.org/10.1111/zsc.12175}, author = {Carbayo, Fernando and Francoy, Tiago M. and Giribet, Gonzalo} } @article {ZOJ:ZOJ12358, title = {The true identity of Obama (Platyhelminthes: Geoplanidae) flatworm spreading across Europe}, journal = {Zoological Journal of the Linnean Society}, volume = {177}, number = {1}, year = {2016}, pages = {5{\textendash}28}, abstract = {

Since 2008 there have been many records in Europe (British Isles, Spain, France, Italy) of a large terrestrial planarian morphologically very similar to the Brazilian species Obama marmorata. Sequences of mitochondrial (Cox1) and nuclear (18S, 28S, ITS-1 and EF) genes from European specimens and some from Brazil indicate that they belong to a species different from that of other specimens also collected in Brazil. Moreover, the phylogenetic results show that they are not sister-species. Histological sections of both Brazilian and European specimens reveal subtle morphological differences between the two species. Obama marmorata is confined to Brazil, and the second, herein described new species, Obama nungara sp. nov., is found in Brazil and Europe. These cryptic species may be syntopic in areas in Brazil. The new species occurs in human-modified environments both in Brazil and in Europe. We also conclude that the specimens from Spain and Argentina identified previously as Obama marmorata belong to the new species.

}, keywords = {BPP, Continenticola, Cox I gene, Geoplana, GMYC, histology, morphology, phylogeny, planarians}, issn = {1096-3642}, doi = {10.1111/zoj.12358}, url = {http://dx.doi.org/10.1111/zoj.12358}, author = {Carbayo, Fernando and {\'A}lvarez-Presas, Marta and Jones, Hugh D. and Riutort, Marta} } @booklet {1547, title = {Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes)}, volume = {5}, year = {2016}, month = {2016//}, pages = {1 - 7}, abstract = {

We investigated whether images obtained through X-ray micro-computed tomography (\μCT) can be used in conjunction with traditional methods for morphological studies of soft-bodied land planarians. \μCT is non-invasive and provides true-to-scale three-dimensional imagery at high resolution. We compared \μCT-based images of a recently described land planarian species of Obama otavioi (Platyhelminthes) with those obtained from light microphotography of histological sections, most of which were also digitized at high magnification.

}, isbn = {2047-217X}, url = {http://dx.doi.org/10.1186/s13742-016-0119-4}, author = {Carbayo, Fernando and Lenihan, Jennifer Winifred} } @article {39, title = {Terrestrial flatworm (Platyhelminthes: Tricladida: Terricola) diversity versus man-induced disturbance in an ombrophilous forest in southern Brazil}, journal = {Biodiversity and Conservation}, volume = {11}, number = {6}, year = {2002}, month = {2002}, pages = {1091-1104}, chapter = {1091}, url = {http://dx.doi.org/10.1023/A:1015865005604}, author = {Carbayo, Fernando and Leal-Zanchet, Ana Maria and Vieira, Emerson M.} } @article {30, title = {Two new genera of geoplaninid land planarians (Platyhelminthes : Tricladida : Terricola) of Brazil in the light of cephalic specialisations}, journal = {Invertebrate Systematics}, volume = {17}, year = {2003}, month = {2003}, pages = {449-468}, chapter = {449}, author = {Carbayo, Fernando and Leal-Zanchet, A. M.} } @article {309, title = {Two new land planarian species (Platyhelminthes: Tricladida) from the Brazilian Atlantic Forest}, journal = {Zoologia (Curitiba)}, volume = {29}, number = {5}, year = {2012}, pages = {430{\textendash}438}, author = {Ana Laura Almeida and Raquel Gualda Kishimoto and Carbayo, Fernando} } @article {153, title = {Land planarians (Platyhelminthes) as a model organism for fine-scale phylogeographic studies: understanding patterns of biodiversity in the Brazilian Atlantic Forest hotspot}, journal = {Journal of Evolutionary Biology}, volume = {24}, number = {4}, year = {2011}, pages = {887-896}, chapter = {887}, abstract = {

The Brazilian Atlantic Forest is one of the richest biodiversity hotspots of the world. Paleoclimatic models have predicted two large stability regions in its northern and central parts, whereas southern regions might have suffered strong instability during Pleistocene glaciations. Molecular phylogeographic and endemism studies show, nevertheless, contradictory results: although some results validate these predictions, other data suggest that paleoclimatic models fail to predict stable rainforest areas in the south. Most studies, however, have surveyed species with relatively high dispersal rates whereas taxa with lower dispersion capabilities should be better predictors of habitat stability. Here, we have used two land planarian species as model organisms to analyse the patterns and levels of nucleotide diversity on a locality within the Southern Atlantic Forest. We find that both species harbour high levels of genetic variability without exhibiting the molecular footprint of recent colonization or population expansions, suggesting a long-term stability scenario. The results reflect, therefore, that paleoclimatic models may fail to detect refugia in the Southern Atlantic Forest, and that model organisms with low dispersal capability can improve the resolution of these models.

}, doi = {10.1111/j.1420-9101.2010.02220.x}, url = {http://onlinelibrary.wiley.com/doi/10.1111/j.1420-9101.2010.02220.x/abstract}, author = {Marta {\'A}lvarez-Presas and Carbayo, Fernando and Julio Rozas and Marta Riutort} } @article {476, title = {Molecular phylogeny of Geoplaninae (Platyhelminthes) challenges current classification: proposal of taxonomic actions}, journal = {Zoologica Scripta}, volume = {42}, number = {5}, year = {2013}, pages = {508-528}, author = {Carbayo, Fernando and Marta {\'A}lvarez-Presas and Olivares, Cl{\'a}udia T. and Marques, Fernando P. L. and Froehlich, Eud{\'o}xia and M. Riutort, Marta} }