@article {587, title = {Genetic Divergence of Japanese Turbellarians, Studied by Comparisons of Partial 18S rRNA Gene Sequences. I. On Representatives of Dendrocoelidae (Platyhelminthes: Tricladida: Paludicola)}, journal = {Zoological Science}, volume = {17}, number = {4}, year = {2000}, pages = {491-496}, abstract = {

This study obtained 18S rDNA sequence data to investigate the extent of genetic divergence in the Japanese dendrocoelid group, and applied the data to the estimation of phylogenetic relationships and taxonomy. The sequence data, consisting of 558 base positions in an aligned data set, were obtained by using the polymerase chain reaction and direct sequencing. For comparative analysis, the 18S rRNA gene sequence data on dendrocoelids from different distant geographical places (Europe, Lake Baikal in Siberia, Kamchatka) published previously were used. Such analysis showed that: (1) Bdellocephala species from Japan, Kamchatka and Lake Baikal are closely related ; (2) Japanese species classified within the genus Dendrocoelopsis do not form a single cluster of closely related organisms; (3) Dendrocoelopsis ichikawai differs minimally from Bdellocephala baicalensis (from Lake Baikal), reliably groups with all Bdellocephala, and should therefore be classed with this genus. Taxonomic conclusions. (1) The genus Dendrocoelopsis should be revised after molecular typification of European and American representatives. (2) The current generic status of D. ichikawai based on the presence of a well-developed penis papilla should be reconsidered. (3) Sequence divergence amongst representatives of B. brunnea indicates that representatives should be closely examined for the presence of morphological characters by which they might be distinguished.

}, doi = {http://dx.doi.org/10.2108/0289-0003(2000)17[491:GDOJTS]2.0.CO;2}, author = {Konstantin D. Kuznedelov and Sachiko Ishida and Shin-ichiro Nishitani} } @article {353, title = {New karyotype of the freshwater planarian Seidlia auriculata collected from shirakami mountains}, journal = {Zoological Science}, volume = {21}, number = {2}, year = {2004}, pages = {1345}, author = {Ishida, S. and Yoshida, W. and Nishitani, S. and Teshirogi, W.} } @article {669, title = {The Dugesia ryukyuensis database as a molecular resource for studying switching of the reproductive system}, journal = {Zoological Science}, volume = {24}, number = {1}, year = {2007}, month = {01/2007}, pages = {31-37}, publisher = {Zoological Society of Japan}, abstract = {

The planarian Dugesia ryukyuensis reproduces both asexually and sexually, and can switch from one mode of reproduction to the other. We recently developed a method for experimentally switching reproduction of the planarian from the asexual to the sexual mode. We constructed a cDNA library from sexualized D. ryukyuensis and sequenced and analyzed 8,988 expressed sequence tags (ESTs). The ESTs were analyzed and grouped into 3,077 non-redundant sequences, leaving 1,929 singletons that formed the basis of unigene sets. Fifty-six percent of the cDNAs analyzed shared similarity (E-value\<1E -20) with sequences deposited in NCBI. Highly redundant sequences encoded granulin and actin, which are expressed in the whole body, and other redundant sequences encoded a Vasa-like protein, which is known to be a component of germ-line cells and is expressed in the ovary, and Y-protein, which is expressed in the testis. The sexualized planarian expressed sequence tag database (http://planaria.bio.keio.ac.jp/planaria/) is an open-access, online resource providing access to sequence, classification, clustering, and annotation data. This database should constitute a powerful tool for analyzing sexualization in planarians.

}, doi = {http://dx.doi.org/10.2108/zsj.24.31}, url = {http://www.bioone.org/doi/abs/10.2108/zsj.24.31}, author = {Hideyuki Ishizuka and Takanobu Maezawa and Junpei Kawauchi and Hanae Nodono and Yukako Hirao and Osamu Nishimura and Haruka Nakagawa and Kiyono Sekii and Kenta Tasaka and Hiroshi Tarui and Kiyokazu Agata and Hoshi, Motonori and Kobayashi, Kazuya and Yasubumi Sakakibara and Matsumoto, Midori} } @article {66, title = {Biogeography and Karyotypes of Freshwater Planarians (Platyhelminthes, Tricladida, Paludicola) in Southern Brazil}, journal = {Zoological Science}, volume = {24}, year = {2007}, pages = {123{\textendash}129}, chapter = {123}, author = {Tanise Knakievicz and Adriana Helena Lau and Daniel Pr{\'a} and Bernardo Erdtmann} } @article {472, title = {Reproductive Strategies, Karyology, Parasites, and Taxonomic Status of Dugesia Populations from Yemen (Platyhelminthes: Tricladida: Dugesiidae)}, journal = {Zoological Science}, volume = {30}, number = {6}, year = {2013}, pages = {502-508}, doi = {http://dx.doi.org/10.2108/zsj.30.502}, url = {http://www.bioone.org/doi/abs/10.2108/zsj.30.502?url_ver=Z39.88-2003\&rfr_id=ori:rid:crossref.org\&rfr_dat=cr_pub\%3dpubmed}, author = {Harrath, Abdul Halim and Ronald Sluys and Waleed Aldahmash and Abdulkarim Al-Razaki and Saleh Alwasel} } @article {482, title = {The Complete Mitochondrial Genome of Dugesia japonica (Platyhelminthes; Order Tricladida)}, journal = {Zoological Science}, volume = {29}, number = {10}, year = {2012}, month = {10/2012}, pages = {672-680}, publisher = {Zoological Society of Japan}, chapter = {672}, abstract = {

We used two sequencing methods, namely long polymerase chain reaction (PCR) and primer walking, to determine the complete mitochondrial DNA (mtDNA) sequence of Dugesia japonica and most of the mtDNA sequence of Dugesia ryukyuensis. The genome of D. japonica contained 36 genes including 12 of the 13 protein-coding genes characteristic of metazoan mitochondrial genomes, two ribosomal RNA genes, and 22 transfer RNA genes. The genome of D. ryukyuensis contained 33 genes, including 12 protein-coding genes, two ribosomal RNA genes, and 19 transfer RNA genes. The gene order of the mitochondrial genome from the Dugesia species showed no clear homology with either the Neodermata or other free-living Rhabditophora. This indicates that the platyhelminths exhibit great variability in mitochondrial gene order. This is the first complete sequence analysis of the mitochondrial genome of a free-living member of Rhabditophora, which will facilitate further studies on the population genetics and genomic evolution of the Platyhelminthes.

}, doi = {http://dx.doi.org/10.2108/zsj.29.672}, url = {http://www.bioone.org/doi/full/10.2108/zsj.29.672}, author = {Masato Sakai and Mitsuru Sakaizumi} }