@article {177, title = {Further Contribution to the Taxonomy of the {\guillemotleft} Dugesia Lugubris-Polychroa Group {\guillemotright} with Description of Dugesia Mediterranea N.SP. (Tricladida, Paludicola)}, journal = {Bolletino di zoologia}, volume = {42}, year = {1975}, note = {doi: 10.1080/11250007509430132}, month = {1975}, pages = {81-89}, publisher = {Taylor \& Francis}, isbn = {0373-4137}, url = {http://www.tandfonline.com/doi/abs/10.1080/11250007509430132}, author = {Mario Benazzi and Jaume Bagu{\~n}{\`a} and Ballester, R. and Puccinelli, I. and Papa, R. D.} } @article {130, title = {The biogeography of the genus Dugesia (Turbellaria, Tricladida, Paludicola) in the Mediterranean region}, journal = {Journal of Biogeography}, volume = {12}, year = {1985}, pages = {509-518}, chapter = {509}, author = {Elizabeth J. De Vries} } @article {21194483, title = {Smed454 dataset: unravelling the transcriptome of Schmidtea mediterranea}, journal = {BMC Genomics}, volume = {11}, number = {1}, year = {2010}, pages = {731}, abstract = {BACKGROUND:Freshwater planarians are an attractive model for regeneration and stem cell research and have become a promising tool in the field of regenerative medicine. With the availability of a sequenced planarian genome, the recent application of modern genetic and high-throughput tools has resulted in revitalized interest in these animals, long known for their amazing regenerative capabilities, which enable them to regrow even a new head after decapitation. However, a detailed description of the planarian transcriptome is essential for future investigation into regenerative processes using planarians as a model system.RESULTS:In order to complement and improve existing gene annotations, we used a 454 pyrosequencing approach to analyze the transcriptome of the planarian species Schmidtea mediterranea Altogether, 598,435 454-sequencing reads, with an average length of 327 bp, were assembled together with the ~10,000 sequences of the S. mediterranea UniGene set using different similarity cutoffs. The assembly was then mapped onto the current genome data. Remarkably, our Smed454 dataset contains more than 3 million novel transcribed nucleotides sequenced for the first time. A descriptive analysis of planarian splice sites was conducted on those Smed454 contigs that mapped univocally to the current genome assembly. Sequence analysis allowed us to identify genes encoding putative proteins with defined structural properties, such as transmembrane domains. Moreover, we annotated the Smed454 dataset using Gene Ontology, and identified putative homologues of several gene families that may play a key role during regeneration, such as neurotransmitter and hormone receptors, homeobox-containing genes, and genes related to eye function.CONCLUSIONS:We report the first planarian transcript dataset, Smed454, as an open resource tool that can be accessed via a web interface. Smed454 contains significant novel sequence information about most expressed genes of S. mediterranea. Analysis of the annotated data promises to contribute to identification of gene families poorly characterized at a functional level. The Smed454 transcriptome data will assist in the molecular characterization of S. mediterranea as a model organism, which will be useful to a broad scientific community.}, issn = {1471-2164}, doi = {10.1186/1471-2164-11-731}, url = {http://www.biomedcentral.com/1471-2164/11/731}, author = {Abril, Josep and Cebria, Francesc and Rodriguez-Esteban, Gustavo and Horn, Thomas and Fraguas, Susanna and Calvo, Beatriz and Bartscherer, Kerstin and Salo, Emili} } @article {400, title = {Molecular markers to characterize the hermaphroditic reproductive system of the planarian Schmidtea mediterranea}, journal = {BMC Developmental Biology}, volume = {11}, year = {2011}, pages = {69}, abstract = {

Background The freshwater planarian Schmidtea mediterranea exhibits two distinct reproductive modes. Individuals of the sexual strain are cross-fertilizing hermaphrodites with reproductive organs that develop post-embryonically. By contrast, individuals of the asexual strain reproduce exclusively by transverse fission and fail to develop reproductive organs. These different reproductive strains are associated with distinct karyotypes, making S. mediterranea a useful model for studying germline development and sexual differentiation. Results To identify genes expressed differentially between these strains, we performed microarray analyses and identified \>800 genes that were upregulated in the sexual planarian. From these, we characterized 24 genes by fluorescent in situ hybridization (FISH), revealing their expression in male germ cells or accessory reproductive organs. To identify additional markers of the planarian reproductive system, we also used immuno- and fluorescent lectin staining, identifying several antibodies and lectins that labeled structures associated with reproductive organs. Conclusions Collectively, these cell-type specific markers will enable future efforts to characterize genes that are important for reproductive development in the planarian.

}, doi = {doi:10.1186/1471-213X-11-69}, url = {http://www.biomedcentral.com/1471-213X/11/69}, author = {Tracy Chong and Joel M Stary and Yuying Wang and Newmark, Phillip A.} } @article {162, title = {Schmidtea mediterranea phylogeography: an old species surviving on a few Mediterranean islands?}, journal = {BMC Evolutionary Biology}, volume = {11}, number = {1}, year = {2011}, chapter = {274}, doi = {10.1186/1471-2148-11-274}, url = {http://www.biomedcentral.com/content/pdf/1471-2148-11-274.pdf}, author = {Eva M. L{\'a}zaro and Harrath, Abdul Halim and Stocchino, Giacinta and Maria Pala and Jaume Bagu{\~n}{\`a} and Marta Riutort} } @article {305, title = {Ultrastructure of spermatogenesis and mature spermatozoon of the freshwater planarian Schmidtea mediterranea (Platyhelminthes, Paludicola)}, journal = {Comptes rendus biologies}, volume = {335}, year = {2012}, pages = {87-95}, author = {Harrath, A. H. and S. Alwasel and Zghal, F. and Tekaya, S.} } @article {758, title = {Clonogenic neoblasts are pluripotent adult stem cells that underlie planarian regeneration}, journal = {Science}, volume = {332}, number = {6031}, year = {2011}, month = {05/2011}, pages = {811-6}, abstract = {

Pluripotent cells in the embryo can generate all cell types, but lineage-restricted cells are generally thought to replenish adult tissues. Planarians are flatworms and regenerate from tiny body fragments, a process requiring a population of proliferating cells (neoblasts). Whether regeneration is accomplished by pluripotent cells or by the collective activity of multiple lineage-restricted cell types is unknown. We used ionizing radiation and single-cell transplantation to identify neoblasts that can form large descendant-cell colonies in vivo. These clonogenic neoblasts (cNeoblasts) produce cells that differentiate into neuronal, intestinal, and other known postmitotic cell types and are distributed throughout the body. Single transplanted cNeoblasts restored regeneration in lethally irradiated hosts. We conclude that broadly distributed, adult pluripotent stem cells underlie the remarkable regenerative abilities of planarians.

}, doi = {10.1126/science.1203983}, url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338249/pdf/nihms355177.pdf}, author = {Daniel E. Wagner and Irving E. Wang and Peter W. Reddien} } @article {167, title = {Freshwater planarians (Platyhelminthes, Tricladida) from the Iberian Peninsula and Greece: diversity and notes on ecology}, journal = {Zootaxa}, volume = {2779}, year = {2011}, month = {02/2011}, pages = {1-38}, author = {Miquel Vila-Farr{\'e} and Ronald Sluys and {\'I}o Almagro and Mette Handberg-Thorsager and Rafael Romero} } @article {308, title = {A new species of Dugesia (Platyhelminthes, Tricladida, Dugesiidae) from the Afromontane forest in South Africa, with an overview of freshwater planarians from the African continent}, journal = {Zootaxa}, volume = {3551}, year = {2012}, pages = {43{\textendash}58}, author = {Giacinta Angela Stocchino and Ronald Sluys and Manconi, Renata} } @article {401, title = {Q\&A: What is regeneration, and why look to planarians for answers?}, journal = {BMC Biology}, volume = {10}, year = {2012}, chapter = {88}, doi = {10.1186/1741-7007-10-88}, url = {http://www.biomedcentral.com/1741-7007/10/88}, author = {Alejandro S{\'a}nchez Alvarado} }