01970nas a2200157   4500008004100000022001400041245011500055210006900170300001400239490000700253520144600260100002201706700002301728700002101751856004001772       2016                        eng d  a1463-640900aNon-destructive imaging to describe a new species of <i>Obama</i> land planarian (Platyhelminthes, Tricladida)0 aNondestructive imaging to describe a new species of iObamai land  a566–5780 v453 a<p>The land planarians in the genus Obama include the largest species of the Neotropical Geoplaninae. Morphological discrimination of Obama species can be difficult, as many species are morphologically similar and some present asymmetric &ndash; difficult to interpret &ndash; copulatory organs. New techniques are thus welcomed to provide faster species description and identification. Here, we study several specimens of Obama by means of mainly 2D and 3D &mu;CT-based images obtained through X-ray microcomputed tomography (&mu;CT) of a paratype, and, complementarily, histological sections of the holotype and a second paratype, which were digitized into virtual slides. Comparison of these images with traditional histological sections and descriptions of the known species of the genus allows us to conclude that our specimens constitute a new species, which we describe here. We further evaluate the phylogenetic placement of the new species using gene fragments from the mitochondrial cytochrome c oxidase subunit I and the nuclear elongation factor-1&alpha;. Raw and derivative &mu;CT data and virtual histological sections were deposited in an open repository (GigaDB) and are freely available. This work leads us to conclude that &mu;CT constitutes a relatively fast, inexpensive non-destructive method that produces results comparable to those of traditional histology, and is thus amenable for describing flatworm species.</p>
1 aCarbayo, Fernando1 aFrancoy, Tiago, M.1 aGiribet, Gonzalo  uhttp://dx.doi.org/10.1111/zsc.12175