01493nas a2200157   4500008004100000245009500041210006900136260001200205300001000217490000800227520095700235100002301192700002101215700002301236856007601259       2011                        eng d00aClonogenic neoblasts are pluripotent adult stem cells that underlie planarian regeneration0 aClonogenic neoblasts are pluripotent adult stem cells that under  c05/2011  a811-60 v3323 a<p>Pluripotent cells in the embryo can generate all cell types, but lineage-restricted cells are generally thought to replenish adult tissues. Planarians are flatworms and regenerate from tiny body fragments, a process requiring a population of proliferating cells (neoblasts). Whether regeneration is accomplished by pluripotent cells or by the collective activity of multiple lineage-restricted cell types is unknown. We used ionizing radiation and single-cell transplantation to identify neoblasts that can form large descendant-cell colonies in vivo. These clonogenic neoblasts (cNeoblasts) produce cells that differentiate into neuronal, intestinal, and other known postmitotic cell types and are distributed throughout the body. Single transplanted cNeoblasts restored regeneration in lethally irradiated hosts. We conclude that broadly distributed, adult pluripotent stem cells underlie the remarkable regenerative abilities of planarians.</p>
1 aWagner, Daniel, E.1 aWang, Irving, E.1 aReddien, Peter, W.  uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338249/pdf/nihms355177.pdf