01431nas a2200145 4500008004100000245014400041210006900185300001200254490000700266520086900273100001801142700002401160700001801184856008301202 1998 eng d00aThe genus Dugesia in Australia, with its phylogenetic analysis and historical biogeography (Platyhelminthes, Tricladida, Dugesiidae)0 agenus iDugesiai in Australia with its phylogenetic analysis and  a273-2890 v273 a

The new species Dugesia notogaea Sluys & Kawakatsu, sp. n. is described from Queensland, Australia, representing the first record of the genus Dugesia for that continent. A phylogenetic analysis of the 68 species of Dugesia resulted in the discovery of a new apomorphy for the genus, and a cladogram showing two major phyletic groups. The distributional range of one group has a western accent, whereas that of the other group has an eastern emphasis; the ranges of these two groups overlap in Africa and the Mediterranean region. We attempt to determine the ancestral area for both the Dugesiidae and Dugesia. A scenario for the historical biogeography of the genus Dugesia is discussed. In this scenario it is presumed that the geological history of the Australasian region enabled Dugesia’s from southeast Asia to colonize northern Australia.

1 aSluys, Ronald1 aKawakatsu, Masaharu1 aWinsor, Leigh uhttp://onlinelibrary.wiley.com/doi/10.1111/j.1463-6409.1998.tb00461.x/abstract02158nas a2200193 4500008004100000245012000041210006900161260001200230300001400242490000800256520150700264100001801771700001801789700002801807700002401835700002001859700001901879856006601898 2013 eng d00aIntegrative delineation of species of Mediterranean freshwater planarians (Platyhelminthes: Tricladida: Dugesiidae)0 aIntegrative delineation of species of Mediterranean freshwater p c11/2013 a523–5470 v1693 a

The paper presents an integrative taxonomic study on dugesiid freshwater flatworms from the north-eastern Mediterranean region by applying both morphological and molecular criteria in the formulation of stable species hypotheses. The morphological information obtained for the specimens was used in a traditional way by comparing the organismal traits of the various populations and candidate species with those of known species, as documented in the taxonomic literature and as revealed by examination of histological sections of museum specimens. In the molecular species delimitation the General Mixed Yule-Coalescent method (GMYC) was used. Results of this study (1) supported the presence of 13 Dugesia species in the Hellenic area (including D. sicula Lepori, 1948, a pan-Mediterranean species), (2) culminated in the description of four new Dugesia species, (3) suggested the presence of two Confirmed Candidate Species, (4) pointed to 12 GMYC-delimited units in Greece and two in Slovakia as Unconfirmed Candidate Species and (5) revealed the presence of an entirely new genus, represented by two newly described species and a third Unconfirmed Candidate Species. Our results revealed a high diversity of dugesiid species in this relatively small region. It is concluded that the morphological features used by taxonomists in comparative studies of dugesiid flatworms generally result in reliable identifications and delineations of species taxa, except in the case of cryptic species.

1 aSluys, Ronald1 aSolà, Eduard1 aGritzalis, Konstantinos1 aVila-Farré, Miquel1 aMateos, Eduardo1 aRiutort, Marta uhttp://onlinelibrary.wiley.com/doi/10.1111/zoj.12077/abstract03182nas a2200277 4500008004100000020001400041245017400055210006900229260001300298300001400311490000700325520228600332653001102618653001702629653000802646653002102654653002602675653001002701653002002711653001902731100001802750700001802768700002802786700001902814856007102833 2013 eng d a1055-790300aFluvial basin history in the northeastern Mediterranean region underlies dispersal and speciation patterns in the genus Dugesia (Platyhelminthes, Tricladida, Dugesiidae)0 aFluvial basin history in the northeastern Mediterranean region u c2013/3// a877 - 8880 v663 a

In this study we analyzed the phylogenetic relationships of eastern Mediterranean freshwater planarians of the genus Dugesia, estimated divergence times for the various clades, and correlated their phylogeographic patterns with geological and paleoclimatic events, in order to discover which evolutionary processes have shaped the present-day distribution of these animals. Specimens were collected from freshwater courses and lakes in continental and insular Greece. Genetic divergences and phylogenetic relationships were inferred by using the mitochondrial gene subunit I of cytochrome oxidase (COI) and the nuclear ribosomal internal transcribed spacer-1 (ITS-1) from 74 newly collected individuals from Greece. Divergence time estimates were obtained under a Bayesian framework, using the COI sequences. Two alternative geological dates for the isolation of Crete from the mainland were tested as calibration points. A clear phylogeographic pattern was present for Dugesia lineages in the Eastern Mediterranean. Morphological data, combined with information on genetic divergences, revealed that eight out of the nine known species were represented in the samples, while additional new, and still undescribed species were detected. Divergence time analyses suggested that Dugesia species became isolated in Crete after the first geological isolation of the island, and that their present distribution in the Eastern Mediterranean has been shaped mainly by vicariant events but also by dispersal. During the Messinian salinity crisis these freshwater planarians apparently were not able to cross the sea barrier between Crete and the mainland, while they probably did disperse between islands in the Aegean Sea. Their dependence on freshwater to survive suggests the presence of contiguous freshwater bodies in those regions. Our results also suggest a major extinction of freshwater planarians on the Peloponnese at the end of the Pliocene, while about 2 Mya ago, when the current Mediterranean climate was established, these Peloponnese populations probably began to disperse again. At the end of the Pliocene or during the Pleistocene, mainland populations of Dugesia colonized the western coast, including the Ionian Islands, which were then part of the continent.

10aAegean10aBiogeography10aCOI10aDivergence times10aFreshwater planarians10aITS-110aMolecular clock10aPhylogeography1 aSolà, Eduard1 aSluys, Ronald1 aGritzalis, Konstantinos1 aRiutort, Marta uhttp://www.sciencedirect.com/science/article/pii/S1055790312004423