01560nas a2200145   4500008004100000245010100041210006900142260004100211300001200252490000700264520104800271100001801319700002301337856005401360       2012                        eng d00aThe Complete Mitochondrial Genome of <i>Dugesia japonica</i> (Platyhelminthes; Order Tricladida)0 aComplete Mitochondrial Genome of iDugesia japonicai Platyhelmint  bZoological Society of Japanc10/2012  a672-6800 v293 a<p>We used two sequencing methods, namely long polymerase chain reaction (PCR) and primer walking, to determine the complete mitochondrial DNA (mtDNA) sequence of Dugesia japonica and most of the mtDNA sequence of Dugesia ryukyuensis. The genome of D. japonica contained 36 genes including 12 of the 13 protein-coding genes characteristic of metazoan mitochondrial genomes, two ribosomal RNA genes, and 22 transfer RNA genes. The genome of D. ryukyuensis contained 33 genes, including 12 protein-coding genes, two ribosomal RNA genes, and 19 transfer RNA genes. The gene order of the mitochondrial genome from the Dugesia species showed no clear homology with either the Neodermata or other free-living Rhabditophora. This indicates that the platyhelminths exhibit great variability in mitochondrial gene order. This is the first complete sequence analysis of the mitochondrial genome of a free-living member of Rhabditophora, which will facilitate further studies on the population genetics and genomic evolution of the Platyhelminthes.</p>
1 aSakai, Masato1 aSakaizumi, Mitsuru  uhttp://www.bioone.org/doi/full/10.2108/zsj.29.672