@article {1893, title = {Planarian regeneration between 1960s and 1990s: From skilful baffled ancestors to bold integrative descendants. A personal account}, pages = { - }, abstract = {AbstractScientific fields grow by accretion of knowledge brought up by succesive generations of scientists. With the field of planarian regeneration as a general background, here I give a personal account of it from the late 1960s until the late 1990s when new research groups, namely Americans, broke into it. After a short historical summary, I report how I got into regeneration, why I choose planarians, and most especially the finding and description of the current model organism Schmidtea mediterranea. Next, I concentrate on the quantitative cellular approaches to regeneration, growth, and degrowth undertaken in our lab in Barcelona, and the long struggle to set neoblasts as a totipotent/pluripotent heterogeneous population of mitotic and non-mitotic cells for homeostatic somatic cell renewal, reproduction, and blastema formation during regeneration. Finally, the first attempts by several labs to analyze regeneration in molecular terms (RNAs, proteins, mAbs, gene detection, cloning and expression) are also covered. Along this essay, I am honoured to pay due tribute to our forebears (ancestors) from Morgan and Child to the French School researchers. In spite of being often baffled by planarian {\textquoteleft}tricks{\textquoteright}, they struggled hard to find new techniques, new ways, and new ideas to tackle the main problems of regeneration, paving the way to those (descendants) that followed suit. }, keywords = {Growth, Neoblast, Pattern formation, planaria, regeneration, S. mediterranea}, isbn = {1084-9521}, url = {https://www.sciencedirect.com/science/article/pii/S1084952117301994}, author = {BAGU{\~N}A, JAUME} } @article {1715, title = {The impairments of neoblast division in regenerating planarian Polycelis felina (Daly.) caused by in vitro treatment with cadmium sulfate}, journal = {Toxicol In Vitro}, volume = {18}, year = {2004}, month = {2004 Feb}, pages = {99-107}, abstract = {

The effects of cadmium sulfate on the neoblast mitotic activity in regenerating planarian Polycelis felina (Daly.) were investigated. Mitotic abnormalities and chromosomal aberrations were evaluated after 6-h treatment and 24-h recovery period. The blastema were fixed, and examined cytologically through routine lactoorceine squash preparations. Mitotic indices were also determined. Cadmium sulfate induced a dose-dependent decrease in neoblast mitotic activity, accompanied with disturbances in distribution of cells over mitotic phases. Different cytological abnormalities with varying frequency were observed. Marked mitotic depression was concentration-dependent. Toxic effects of cadmium in regenerating planarian were mainly associated with mitotic spindle disturbances. Immediately after treatment mitotic abnormalities were prevalent over chromosomal and C-mitosis was the most prominent one. After 24-h recovery period a prevalence of mitotic over chromosomal aberrations was still present in animals treated with two higher concentrations of cadmium sulfate. However, the proportions of cells with chromosome stickiness in all treated animals were significantly increased compared to their post-treatment values. Observed mitotic impairments could be related to mitotic arrest contributing to retardations and delays, especially in animals treated with the highest concentration tested. The results obtained indicated usefulness of short term invertebrate assays as an alternative to in vitro pre-screening of toxic chemicals.

}, keywords = {Animals, Biological Assay, Cadmium Compounds, Cell Division, Chromosome Aberrations, Croatia, Dose-Response Relationship, Drug, Drug Evaluation, Preclinical, Female, Forecasting, Mitosis, Mitotic Index, Mortality, Oxygen Consumption, planarians, regeneration, Sulfates, Survival, Time Factors}, issn = {0887-2333}, author = {Kalafati{\'c}, M and Kopjar, N and Besendorfer, V} } @article {1710, title = {On the distribution of neoblasts of Dugesia lugubris (Turbellaria, Tricladida) before and during regeneration}, journal = {C R Hebd Seances Acad Sci}, volume = {250}, year = {1960}, month = {1960 Mar 28}, pages = {2465-7}, keywords = {Animals, Annelida, Asteraceae, planarians, Platyhelminths, regeneration, Turbellaria}, issn = {0001-4036}, author = {LENDER, T and GABRIEL, A} } @article {1703, title = {Genes for regeneration}, journal = {eLife}, volume = {3}, year = {2014}, month = {2014/04/15}, pages = {e02517}, publisher = {eLife Sciences Publications, Ltd}, abstract = {FoxA, an evolutionarily conserved gene involved in the development of the digestive system in many animals, has an important role in regeneration in flatworms.}, keywords = {Pharynx, planaria, regeneration, single-organ regeneration, stem cell}, isbn = {2050-084X}, url = {https://dx.doi.org/10.7554/eLife.02517}, author = {Rossant, Janet} }