@article {1719,
	title = {Resistance of two planarian species to UV-irradiation},
	journal = {Folia Biol (Krakow)},
	volume = {54},
	year = {2006},
	month = {2006},
	pages = {103-8},
	abstract = {<p>The aim of this work was to determine the effects of 20, 25 and 30 minute UV-irradiation periods lambda = 253.5 nm to two planarian species Dugesia tigrina (Gir.) and Polycelis felina (Daly.). In vivo, UV light effects have been reported to affect intracellular receptors and disrupt simple behaviour. The effects of UV-rays on mortality and behavior as well as morphological, cytological and histological changes in the two planarian species were assessed, and the course and the dynamics of regenerative processes were compared between them. Experimental populations of Dugesia tigrina and Polycelis felina species were maintained in laboratory conditions at room temperature. Mortality, behavioral and morphological changes were monitored daily by means of a light stereomicroscope. For cytological and histopathological analysis, planarians were fixed in Bouine fixative on the first, second, third, fifth and seventh day after exposure to UV-irradiation, respectively. They were embedded in paraffin, cut on a microtome, stained with toluidin blue and embedded in Canada-balsam. UV-rays caused mortality, behavioral, morphological, cytological and histological changes in each planarian species. In regeneration of damaged body parts reticular cells and neoblasts played the main role. Neoblasts as totipotent cells extremely increased in number in the area of damaged tissue, immediately after UV-exposure. Dugesia tigrina was more sensitive to UV-rays than Polycelis felina due to possession of less pigmented cells. The course of regeneration in both species was similar. Most individuals of both species regenerated in 5 to 12 days after UV-irradiation.</p>
},
	keywords = {Animals, planarians, Ultraviolet Rays},
	issn = {0015-5497},
	author = {Kalafati{\'c}, Mirjana and Kovacevi{\'c}, Goran and Franjevi{\'c}, Damjan}
}
@article {1718,
	title = {Toxicity of nitrite to three species of freshwater invertebrates.},
	journal = {Environ Toxicol},
	volume = {21},
	year = {2006},
	month = {2006 Feb},
	pages = {90-4},
	abstract = {<p>Nitrite is a compound with a high toxicity to aquatic animals. Several anthropogenic pollution sources are increasing the concentrations of this component of the nitrogen cycle. Despite this toxicity, there is little available literature on its effects on freshwater invertebrates. Laboratory bioassays were performed to obtain data on the lethal effects of nitrite to three species of freshwater invertebrates: the planarian Polycelis felina and the amphipods Echinogammarus echinosetosus and Eulimnogammarus toletanus. The LC(50), LC(10), and LC(0.01) values (mg/L NO(2)--N) at 24, 48, 72, and 96 h were calculated for each species. E. toletanus and E. echinosetosus were the most sensitive species, with 96 h LC(50) values of 2.09 and 2.59 mg/L NO(2)--N, respectively. In contrast, the planarian P. felina showed a higher tolerance to nitrite, with a 96 h LC(50) value of 60.0 mg/L NO(2)--N. The obtained results were compared with the reported nitrite data for other freshwater invertebrates. This study may contribute to a more appropriate assessment of the ecological risk of this compound in freshwater ecosystems.</p>
},
	keywords = {Amphipoda, Animals, Lethal Dose 50, Nitrites, planarians, Rivers, Spain, Water Pollutants, Chemical},
	issn = {1520-4081},
	doi = {10.1002/tox.20155},
	author = {Alonso, Alvaro and Camargo, Julio A}
}
