Publication Type: | Journal Article |
Year of Publication: | 1995 |
Authors: | A. Hutticher, Kerschbaum, H. H., Kainz, V., Bito, M., Hermann, A. |
Journal: | Cell Mol Neurobiol |
Volume: | 15 |
Issue: | 4 |
Pagination: | 401-10 |
Date Published: | 1995 Aug |
ISSN: | 0272-4340 |
Keywords: | Animals, Blotting, Western, Calcium-Binding Proteins, Immunohistochemistry, Molecular Weight, Nervous system, Parvalbumins, planarians |
Abstract: | 1. Using immunological methods, we have identified parvalbumin-like material in the triclade flatworms, Polycelis nigra, Polycelis auriculata, Crenobia alpina, Dugesia tahitiensis, and Dugesia polychroa. 2. Western immunoblot analysis of these five species revealed heat stable parvalbumin-immunoreactive proteins between 32 kD and 44 kD. 3. Proteins at 19 and 32 kD revealed intense labeling with 45Ca2+. 4. Double immunodiffusion of planarian supernatants showed complete fusion of precipitates, indicating immunological relatedness of the parvalbumin-like material among the species investigated. 5. Immunocytochemical studies exhibit parvalbumin-immunoreactive material exclusively in neurons supporting the notion of an early evolutionary appearance of these proteins in the nervous system. |
Alternate Journal: | Cell. Mol. Neurobiol. |
Parvalbumin-immunoreactive proteins in the nervous system of planarians
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