Parvalbumin-immunoreactive proteins in the nervous system of planarians

Publication Type:Journal Article
Year of Publication:1995
Authors:A. Hutticher, Kerschbaum, H. H., Kainz, V., Bito, M., Hermann, A.
Journal:Cell Mol Neurobiol
Volume:15
Issue:4
Pagination:401-10
Date Published:1995 Aug
ISSN:0272-4340
Keywords:Animals, Blotting, Western, Calcium-Binding Proteins, Immunohistochemistry, Molecular Weight, Nervous system, Parvalbumins, planarians
Abstract:

1. Using immunological methods, we have identified parvalbumin-like material in the triclade flatworms, Polycelis nigra, Polycelis auriculata, Crenobia alpina, Dugesia tahitiensis, and Dugesia polychroa. 2. Western immunoblot analysis of these five species revealed heat stable parvalbumin-immunoreactive proteins between 32 kD and 44 kD. 3. Proteins at 19 and 32 kD revealed intense labeling with 45Ca2+. 4. Double immunodiffusion of planarian supernatants showed complete fusion of precipitates, indicating immunological relatedness of the parvalbumin-like material among the species investigated. 5. Immunocytochemical studies exhibit parvalbumin-immunoreactive material exclusively in neurons supporting the notion of an early evolutionary appearance of these proteins in the nervous system.

Alternate Journal:Cell. Mol. Neurobiol.
Mon, 2017-03-27 04:21 -- Eduard
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